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Inverted microscopes are generally used in the Life Sciences for
examining living specimens. The design enables specimens to be observed from
underneath. They are frequenty used in micromanipulation work, because of the
working distance of the condenser, now situated above the specimen, can be very
long in comparison to the objective's working distance.
Inverted microscopes, used in the life sciences, divide broadly into three
categories, routine for typically looking at tissue cultures, large
research with the possibility of fluorescence and interference and multiple
image ports and intermediate, frequently offering an economical 'half way
house' between the routine and large research.
See below for useful tips
USEFUL TIPS IN
RELATION TO INVERTED MICROSCOPES
| CONDENSERS It is not recommended to
automatically select the longest working distance condenser available.
Select the condenser with the minimum working distance, which is useable
for the application. The longer the condenser working distance, the
usually smaller is its numerical aperture. Larger numerical aperture
condensers will give more resolution with higher magnification
objectives |
OBJECTIVES Longer working distance
objectives are required on inverted microscopes to focus into special
chambers. Such objectives are usually corrected to view through thicker
walls than the thickness of a standard cover slip. On routine models the
objectives are usually corrected for an average chamber thickness e.g.
1mm. On more sophisticated inverted microscopes, the objectives can have
an adjustable 'cover glass' correction. Ensure this is correctly
adjusted for the chamber being used. An incorrect setting can
considerably degrade the image quality |
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